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Following the capture step, semaglutide in 0.1 M HEPES, 1.5 M NaCl, 0.03 M EDTA and 0.5% v/v Surfactant P20, pH 7.4, was injected at concentrations of 1, 2.5, 5, 10 and 25 nM at 25°C and a flowrate of 50 µL/min